A platform for efficient genotyping in Musa using microsatellite markers.

TitleA platform for efficient genotyping in Musa using microsatellite markers.
Publication TypeJournal Article
Year of Publication2011
AuthorsChristelová P, Valárik M, Hřibová E, Van den Houwe I, Channelière S, Roux N, Doležel J
JournalAoB Plants
Volume2011
Paginationplr024
Date Published2011
ISSN2041-2851
Abstract

BACKGROUND AND AIMS: Bananas and plantains (Musa spp.) are one of the major fruit crops worldwide with acknowledged importance as a staple food for millions of people. The rich genetic diversity of this crop is, however, endangered by diseases, adverse environmental conditions and changed farming practices, and the need for its characterization and preservation is urgent. With the aim of providing a simple and robust approach for molecular characterization of Musa species, we developed an optimized genotyping platform using 19 published simple sequence repeat markers.

METHODOLOGY: The genotyping system is based on 19 microsatellite loci, which are scored using fluorescently labelled primers and high-throughput capillary electrophoresis separation with high resolution. This genotyping platform was tested and optimized on a set of 70 diploid and 38 triploid banana accessions.

PRINCIPAL RESULTS: The marker set used in this study provided enough polymorphism to discriminate between individual species, subspecies and subgroups of all accessions of Musa. Likewise, the capability of identifying duplicate samples was confirmed. Based on the results of a blind test, the genotyping system was confirmed to be suitable for characterization of unknown accessions.

CONCLUSIONS: Here we report on the first complex and standardized platform for molecular characterization of Musa germplasm that is ready to use for the wider Musa research and breeding community. We believe that this genotyping system offers a versatile tool that can accommodate all possible requirements for characterizing Musa diversity, and is economical for samples ranging from one to many accessions.

DOI10.1093/aobpla/plr024
Alternate JournalAoB Plants
PubMed ID22476494
PubMed Central IDPMC3185971
Showing 39 of 39 accessions
Accession Name Pictures Accession number Species / Group SubSpecies / SubGroup Origin Collection Available for distribution
Musa ornata ITC0370 ornata subsp. ornata Unknown ITC
Balbisiana ITC0545 balbisiana Unknown Unknown ITC
Tuu Gia ITC0610 AA Unknown Unknown ITC
Long Tavoy ITC0093 acuminata subsp. burmannica Unknown ITC
Calcutta 4 ITC0249 acuminata subsp. burmannicoides India ITC
Musa textilis ITC1072 textilis subsp. textilis Unknown ITC
Musa mannii ITC1411 Unknown Unknown Unknown ITC
Musa beccarii ITC1070 beccarii subsp. beccarii Unknown ITC
Higa ITC0428 acuminata subsp. banksii Unknown ITC
Malaccensis ITC0250 acuminata subsp. malaccensis Unknown ITC
Balbisiana (10852) ITC0094 balbisiana Unknown Unknown ITC
Tavoy ITC0072 acuminata subsp. burmannica Unknown ITC
Tani ITC1120 balbisiana Unknown Unknown ITC
Cameroun ITC0246 balbisiana Unknown Unknown ITC
Tonton Kepa ITC0822 AS Unknown Papua New Guinea ITC
Ato ITC0820 AS Unknown Papua New Guinea ITC
Musa acuminata ssp. banksii ITC0896 acuminata subsp. banksii Papua New Guinea ITC
Musa coccinea ITC0287 coccinea subsp. coccinea Unknown ITC
Menei ITC1021 Fe'i Unknown Papua New Guinea ITC
Musa peekelii ssp. peekelii ITC0917 peekelii subsp. peekelii Papua New Guinea ITC
Musa velutina ITC0638 velutina subsp. velutina Unknown ITC
Pa (Rayong) ITC0672 acuminata subsp. siamea Thailand ITC
Musa velutina ITC0011 velutina subsp. velutina Unknown ITC
Galeo
ITC0259 AA Unknown Unknown ITC
Niyarma Yik
ITC0269 AA Unknown Unknown ITC
Guyod
ITC0299 AA Unknown Philippines ITC
Musa acuminata spp. banksii x Musa schizocarpa
ITC0859 acuminata x schizocarpa Unknown Papua New Guinea ITC
Singapuri
ITC0248 balbisiana Unknown Unknown ITC
Butuhan, salient apex
ITC0564 balbisiana Unknown Philippines ITC
Butuhan
ITC1074 balbisiana x textilis Unknown Unknown ITC
Kawaputa
ITC0927 Fe'i Unknown Papua New Guinea ITC
Musa maclayi
ITC1207 maclayi subsp. maclayi Papua New Guinea ITC
Musa maclayi type Hung Si
ITC0614 maclayi subsp. maclayi Papua New Guinea ITC
Musa ornata (red fingers)
ITC1330 ornata subsp. ornata Unknown ITC
Kluai Bou
ITC0528 ornata subsp. ornata Unknown ITC
Musa mannii H. Wendl
ITC0543 sanguinea subsp. sanguinea Unknown ITC
Schizocarpa
ITC0846 schizocarpa Unknown Papua New Guinea ITC
Musa textilis
ITC0539 textilis subsp. textilis Unknown ITC
Maia Oa
ITC0728 acuminata subsp. zebrina Unknown ITC