Molecular and Cytogenetic Study of East African Highland Banana

TitleMolecular and Cytogenetic Study of East African Highland Banana
Publication TypeJournal Article
Year of Publication2018
AuthorsNěmečková A, Christelová P, Čížková J, Nyine M, Van den houwe I, Svačina R, Uwimana B, Swennen R, Doležel J, Hřibová E
JournalFrontiers in Plant Science
Volume9
Pagination1371
ISSN1664-462X
Abstract

East African highland bananas (EAHB) are staple food crop in Uganda, Tanzania, Burundi and other countries in the African Great Lakes region in Africa. Even though several morphologically different types exist, all EAHB are triploidandtriploid and display minimal genetic variation. To provide more insights into the genetic variation within EAHB, genotyping using simple sequence repeat (SSR) markers, molecular analysis of ITS1-5.8S-ITS2 region of ribosomal DNA locus and the analysis of chromosomal distribution of ribosomal DNA sequences was done. A total of 40 triploid EAHB accessions that were available in the Musa germplasm collection (International Transit Centre, Leuven, Belgium) were characterized. Six diploid accessions of M.Musa acuminata ssp. zebrina, ssp. banksii and ssp. malaccensis representing putative parents of EAHB M. acuminata ssp. zebrina, ssp. banksii and ssp. malaccensis were included in the study. Flow cytometric estimation of 2C nuclear DNA content revealed small differences (max   6.5 %) in genome size among the EAHB clones. While no differencedifferences in the number of 45S and 5S rDNA loci waswere found, genotyping using 19 SSR markers resulted in grouping the EAHB accessions into four clusters. DNA sequence analysis of the ITS region suggestedindicated a relation of EAHB clones with MusaM. acuminata and, surprisingly, also with M. schizocarpa. The results suggest that EAHB cultivars originated from a single hybrid clone with M. acuminata ssp. zebrina and ssp. banksii being its most probable parents. However, M. schizocarpa seems to have contributed to the formation of this group of banana.

URLhttps://www.frontiersin.org/article/10.3389/fpls.2018.01371
DOI10.3389/fpls.2018.01371
Showing 40 of 44 accessions
Accession Name Pictures Accession number Species / Group SubSpecies / SubGroup Origin Collection Available for distribution
Zebrina ITC1177 acuminata subsp. zebrina Unknown ITC
Igihuni ITC0158 AAA subgr. Mutika/Lujugira Unknown ITC
Bui Se-ed ITC0301 AAA subgr. Mutika/Lujugira Indonesia ITC
Naine de Nyangezi ITC0147 AAA subgr. Mutika/Lujugira Unknown ITC
Nshika ITC0145 AAA subgr. Mutika/Lujugira Unknown ITC
Mbirabire ITC0154 AAA subgr. Mutika/Lujugira Unknown ITC
Nakitengwa ITC1180 AAA subgr. Mutika/Lujugira Unknown ITC
Nante ITC1353 AAA subgr. Mutika/Lujugira Unknown ITC
Makara ITC0177 AAA subgr. Mutika/Lujugira Unknown ITC
Guineo ITC0005 AAA subgr. Mutika/Lujugira Unknown ITC
Kitawira ITC0137 AAA subgr. Mutika/Lujugira Unknown ITC
Gashulie ITC0149 AAA subgr. Mutika/Lujugira Unknown ITC
Rugondo ITC0164 AAA subgr. Mutika/Lujugira Unknown ITC
Imbogo ITC0168 AAA subgr. Mutika/Lujugira Unknown ITC
Kikundi ITC1224 AAA subgr. Mutika/Lujugira Tanzania ITC
Kazirakwe ITC1355 AAA subgr. Mutika/Lujugira Unknown ITC
Kagera ITC0141 AAA subgr. Mutika/Lujugira Unknown ITC
Ingarama ITC0160 AAA subgr. Mutika/Lujugira Unknown ITC
N'Dundu ITC0732 AAA subgr. Mutika/Lujugira Unknown ITC
Nakitengwa ITC0085 AAA subgr. Mutika/Lujugira Burundi ITC
Indemera y'Imbihire ITC0161 AAA subgr. Mutika/Lujugira Unknown ITC
Ikimaga ITC0171 AAA subgr. Mutika/Lujugira Unknown ITC
Ingumba y'Imbihire ITC0155 AAA subgr. Mutika/Lujugira Unknown ITC
Inyoya ITC0163 AAA subgr. Mutika/Lujugira Unknown ITC
Ingagara ITC0166 AAA subgr. Mutika/Lujugira Unknown ITC
Ikigeregere ITC0169 AAA subgr. Mutika/Lujugira Unknown ITC
Nyamwihogora ITC0086 AAA subgr. Mutika/Lujugira Burundi ITC
Kokopo ITC1243 AA Unknown Papua New Guinea ITC
Igitsiri (Intuntu) ITC0081 AAA subgr. Mutika/Lujugira Burundi ITC
Igisahira Gisanzwe (Inyamunyu) ITC0083 AAA subgr. Mutika/Lujugira Burundi ITC
Zebrina ITC1139 acuminata subsp. zebrina Unknown ITC
Intama ITC0153 AAA subgr. Mutika/Lujugira Unknown ITC
Mbwazirume ITC1356 AAA subgr. Mutika/Lujugira Unknown ITC
Malaccensis
ITC0074 acuminata subsp. malaccensis Unknown ITC
Ndyali
ITC1552 AA subgr. Mshare Tanzania ITC
Ingumba y'Inyamunyo
ITC0126 AAA subgr. Mutika/Lujugira Unknown ITC
Inzirabahima
ITC0150 AAA subgr. Mutika/Lujugira Unknown ITC
Nyamahwa
ITC1555 AAA subgr. Mutika/Lujugira Tanzania ITC
Nyitabunyonyi
ITC1556 AAA subgr. Mutika/Lujugira Tanzania ITC
Muhongoroka
ITC0156 AAA subgr. Mutika/Lujugira Unknown ITC

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