Development and assessment of Diversity Arrays Technology for high-throughput DNA analyses in Musa.

TitleDevelopment and assessment of Diversity Arrays Technology for high-throughput DNA analyses in Musa.
Publication TypeJournal Article
Year of Publication2009
AuthorsRisterucci A-M, Hippolyte I, Perrier X, Xia L, Caig V, Evers M, Huttner E, Kilian A, Glaszmann J-C
JournalTheor Appl Genet
Volume119
Issue6
Pagination1093-103
Date Published2009 Oct
ISSN1432-2242
KeywordsBiological Evolution, Diploidy, DNA, Plant, Genetic Markers, Genetic Variation, Genome, Plant, Genotype, Guadeloupe, Hybridization, Genetic, Musa, Nigeria, Oligonucleotide Array Sequence Analysis, Polymorphism, Genetic, Polyploidy, Sequence Analysis, DNA, Species Specificity
Abstract

Diversity Arrays Technology (DArT) is a DNA hybridisation-based molecular marker technique that can detect simultaneously variation at numerous genomic loci without sequence information. This efficiency makes it a potential tool for a quick and powerful assessment of the structure of germplasm collections. This article demonstrates the usefulness of DArT markers for genetic diversity analyses of Musa spp. genotypes. We developed four complexity reduction methods to generate DArT genomic representations and we tested their performance using 48 reference Musa genotypes. For these four complexity reduction methods, DArT markers displayed high polymorphism information content. We selected the two methods which generated the most polymorphic genomic representations (PstI/BstNI 16.8%, PstI/TaqI 16.1%) to analyze a panel of 168 Musa genotypes from two of the most important field collections of Musa in the world: Cirad (Neufchateau, Guadeloupe), and IITA (Ibadan, Nigeria). Since most edible cultivars are derived from two wild species, Musa acuminata (A genome) and Musa balbisiana (B genome), the study is restricted mostly to accessions of these two species and those derived from them. The genomic origin of the markers can help resolving the pedigree of valuable genotypes of unknown origin. A total of 836 markers were identified and used for genotyping. Ten percent of them were specific to the A genome and enabled targeting this genome portion in relatedness analysis among diverse ploidy constitutions. DArT markers revealed genetic relationships among Musa genotype consistent with those provided by the other markers technologies, but at a significantly higher resolution and speed and reduced cost.

DOI10.1007/s00122-009-1111-5
Alternate JournalTheor. Appl. Genet.
PubMed ID19693484
Showing 10 of 50 accessions
Accession Name Pictures Accession number Species / Group SubSpecies / SubGroup Origin Collection Available for distribution
Pisang Kayu
ITC0420 AAA subgr. Orotava Indonesia ITC
Nzizi
ITC0334 ABB subgr. Peyan Unknown ITC
Simili Radjah
PT-BA-00369 ABB subgr. Peyan Unknown CIRAD
Gabah Gabah
ITC0307 AA subgr. Pisang Jari Buaya Unknown ITC
Pisang Kelat
ITC0842 AAB subgr. Pisang Kelat Unknown ITC
Lady Finger (Nelson)
ITC0582 AAB subgr. Pome Unknown ITC
Saba
ITC1138 ABB subgr. Saba Unknown ITC
Orishele
ITC1325 AAB subgr. Plantain Nigeria ITC
Banane serpent
ITC0282 AAB subgr. Plantain Unknown ITC
Red Yade
ITC1140 AAB subgr. Plantain Unknown ITC

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