Screening the banana biodiversity for drought tolerance: can an in vitro growth model and proteomics be used as a tool to discover tolerant varieties and understand homeostasis.

TitleScreening the banana biodiversity for drought tolerance: can an in vitro growth model and proteomics be used as a tool to discover tolerant varieties and understand homeostasis.
Publication TypeJournal Article
Year of Publication2012
AuthorsVanhove A-C, Vermaelen W, Panis B, Swennen R, Carpentier SC
JournalFront Plant Sci
Volume3
Pagination176
Date Published2012
ISSN1664-462X
Abstract

There is a great need for research aimed at understanding drought tolerance, screening for drought tolerant varieties and breeding crops with an improved water use efficiency. Bananas and plantains are a major staple food and export product with a worldwide production of over 135 million tonnes per year. Water however is the most limiting abiotic factor in banana production. A screening of the Musa biodiversity has not yet been performed. We at KU Leuven host the Musa International Germplasm collection with over 1200 accessions. To screen the Musa biodiversity for drought tolerant varieties, we developed a screening test for in vitro plants. Five varieties representing different genomic constitutions in banana (AAAh, AAA, AAB, AABp, and ABB) were selected and subjected to a mild osmotic stress. The ABB variety showed the smallest stress induced growth reduction. To get an insight into the acclimation and the accomplishment of homeostasis, the leaf proteome of this variety was characterized via 2D DIGE. After extraction of the leaf proteome of six control and six stressed plants, 2600 spots could be distinguished. A PCA analysis indicates that control and stressed plants can blindly be classified based on their proteome. One hundred and twelve proteins were significantly more abundant in the stressed plants and 18 proteins were significantly more abundant in control plants (FDR α 0.05). Twenty four differential proteins could be identified. The proteome analysis clearly shows that there is a new balance in the stressed plants and that the respiration, metabolism of ROS and several dehydrogenases involved in NAD/NADH homeostasis play an important role.

DOI10.3389/fpls.2012.00176
Alternate JournalFront Plant Sci
PubMed ID22876254
PubMed Central IDPMC3410380
Showing 5 of 5 accessions
Accession Name Pictures Accession number Species / Group SubSpecies / SubGroup Origin Collection Available for distribution
Cachaco ITC0643 ABB subgr. Bluggoe Unknown ITC
Williams ITC0365 AAA subgr. Cavendish Unknown ITC
Mbwazirume ITC0084 AAA subgr. Mutika/Lujugira Burundi ITC
Obino l'Ewai ITC0109 AAB subgr. Plantain Unknown ITC
Popoulou
ITC0335 AAB subgr. Maia Maoli/Popoulu Unknown ITC
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