Molecular and Cytogenetic Characterization of Wild Musa Species.

TitleMolecular and Cytogenetic Characterization of Wild Musa Species.
Publication TypeJournal Article
Year of Publication2015
AuthorsČížková J, Hřibová E, Christelová P, Van den Houwe I, Häkkinen M, Roux N, Swennen R, Doležel J
JournalPLoS One
Volume10
Issue8
Paginatione0134096
Date Published2015
ISSN1932-6203
KeywordsCell Nucleus, Chromosomes, Plant, Cytogenetic Analysis, DNA, Intergenic, DNA, Plant, DNA, Ribosomal, Flow Cytometry, Genome Size, Genotype, In Situ Hybridization, Fluorescence, Microsatellite Repeats, Musa, Phylogeny, Pseudogenes, RNA, Ribosomal, Species Specificity
Abstract

<p>The production of bananas is threatened by rapid spreading of various diseases and adverse environmental conditions. The preservation and characterization of banana diversity is essential for the purposes of crop improvement. The world's largest banana germplasm collection maintained at the Bioversity International Transit Centre (ITC) in Belgium is continuously expanded by new accessions of edible cultivars and wild species. Detailed morphological and molecular characterization of the accessions is necessary for efficient management of the collection and utilization of banana diversity. In this work, nuclear DNA content and genomic distribution of 45S and 5S rDNA were examined in 21 diploid accessions recently added to ITC collection, representing both sections of the genus Musa. 2C DNA content in the section Musa ranged from 1.217 to 1.315 pg. Species belonging to section Callimusa had 2C DNA contents ranging from 1.390 to 1.772 pg. While the number of 45S rDNA loci was conserved in the section Musa, it was highly variable in Callimusa species. 5S rRNA gene clusters were found on two to eight chromosomes per diploid cell. The accessions were genotyped using a set of 19 microsatellite markers to establish their relationships with the remaining accessions held at ITC. Genetic diversity done by SSR genotyping platform was extended by phylogenetic analysis of ITS region. ITS sequence data supported the clustering obtained by SSR analysis for most of the accessions. High level of nucleotide diversity and presence of more than two types of ITS sequences in eight wild diploids pointed to their origin by hybridization of different genotypes. This study significantly expands the number of wild Musa species where nuclear genome size and genomic distribution of rDNA loci is known. SSR genotyping identified Musa species that are closely related to the previously characterized accessions and provided data to aid in their classification. Sequence analysis of ITS region provided further information about evolutionary relationships between individual accessions and suggested that some of analyzed accessions were interspecific hybrids and/or backcross progeny.</p>

DOI10.1371/journal.pone.0134096
Alternate JournalPLoS ONE
PubMed ID26252482
PubMed Central IDPMC4529165
Showing 21 of 21 accessions
Accession Name Pictures Accession number Species / Group SubSpecies / SubGroup Origin Collection Available for distribution
Musa lutea ITC1515 Unknown Unknown Unknown ITC
Musa Beccarii
ITC1516 beccarii Unknown Unknown ITC
Musa beccarii var. Hottana
ITC1529 beccarii Unknown Malaysia ITC
Musa borneensis
ITC1531 borneensis Unknown Malaysia ITC
Musa exotica
ITC1532 Unknown Unknown Vietnam ITC
Musa barioensis
ITC1568 Unknown Unknown Unknown ITC
Musa campestris var. Sarawakensis
ITC1517 campestris Unknown Malaysia ITC
Musa campestris var. Limbangensis
ITC1535 campestris Unknown Brunei ITC
Musa itinerans var. itinerans
ITC1571 Unknown Unknown Unknown ITC
Musa yunnanensis
ITC1573 Unknown Unknown Unknown ITC
Musa itinerans var. Xishuangbannaensis
ITC1526 itinerans Unknown China ITC
Musa laterita
ITC1575 laterita Unknown Unknown ITC
Musa mannii
ITC1574 mannii Unknown Unknown ITC
Musa monticola
ITC1528 monticola Unknown Malaysia ITC
Musa rubinea
ITC1518 Unknown Unknown China ITC
M. x fennicae
ITC1522 Unknown Unknown Finland ITC
Musa siamensis
ITC1534 Unknown Unknown Thailand ITC
Musa rosea x siamensis
ITC1592 Unknown Unknown Unknown ITC
Musa rosea-hybrid
ITC1598 rosea Unknown Unknown ITC
Musa rubra
ITC1590 rubra Unknown Unknown ITC
Musa violascens
ITC1514 violascens Unknown Malaysia ITC