Effects of In Vitro Polyploidization on Agronomic Characteristics and Fruit Carotenoid Content; Implications for Banana Genetic Improvement

TitleEffects of In Vitro Polyploidization on Agronomic Characteristics and Fruit Carotenoid Content; Implications for Banana Genetic Improvement
Publication TypeJournal Article
Year of Publication2019
AuthorsAmah D, van Biljon A, Maziya-Dixon B, Labuschagne M, Swennen R
JournalFrontiers in Plant Science
Volume10
ISSN1664-462X
Keywordsbanana genetic improvement, biofortification, Carotenoid content, Diploid bananas, In vitro polyploidization, Pollen viability, Vitamin A
Abstract

Bananas (Musa spp.), native to South East Asia, have spread worldwide and are integrated into the diets of millions of people in tropical regions. Carotenoid content varies dramatically between different banana genotypes, providing an opportunity for vitamin A biofortification. Polyploidization is a useful tool for crop improvement with potential to generate new diversity, especially in a polyploid crop like bananas. Ten induced tetraploids generated from six diploid banana genotypes were evaluated for their agronomic attributes and fruit carotenoid content in comparison to their diploid progenitors. Tetraploids had distinct plant morphology, but generally displayed inferior vegetative and yield characteristics with 20% lower bunch weights than their original diploids. Similarly, a 50% decrease in fruit provitamin A carotenoids (α-carotene, 13-cis β-carotene, 9-cis β-carotene, trans β-carotene) accompanied by a corresponding increase in lutein was recorded in induced tetraploids in comparison to their original diploids. Additionally, all lines were subjected to pollen viability tests to assess their fertility. Pollen viability tests indicated over 70% viability for induced tetraploids and diploid controls, suggesting their possible use in crosses. These findings provide a basis for the application of induced polyploidization in bananas to generate useful genetic material for integration in hybridization programmes aiming to produce vitamin A enriched triploids valuable to malnourished populations.

URLhttps://www.frontiersin.org/articles/10.3389/fpls.2019.01450/full
DOI10.3389/fpls.2019.01450
Showing 5 of 5 accessions
Accession Name Pictures Accession number Species / Group SubSpecies / SubGroup Origin Collection Available for distribution
Sowmuk ITC0266 AA Unknown Unknown ITC
Pisang Madu ITC0507 AA Unknown Unknown ITC
AAcv Rose ITC0712 AA Unknown Indonesia ITC
Beram ITC0298 AA Unknown Indonesia ITC
Galeo
ITC0259 AA Unknown Unknown ITC
Evaluation traits: