Annotation of differentially expressed genes in the somatic embryogenesis of musa and their location in the banana genome.

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TitleAnnotation of differentially expressed genes in the somatic embryogenesis of musa and their location in the banana genome.
Publication TypeJournal Article
Year of Publication2013
AuthorsMaldonado-Borges JInes, Ku-Cauich JRoberto, Escobedo-Graciamedrano RMaria
Date Published2013
KeywordsChromosome Mapping, Chromosomes, Plant, Gene Expression Regulation, Developmental, Genome, Plant, Molecular Sequence Annotation, Musa, Plant Somatic Embryogenesis Techniques, Seeds

<p>Analysis of cDNA-AFLP was used to study the genes expressed in zygotic and somatic embryogenesis of Musa acuminata Colla ssp. malaccensis, and a comparison was made between their differential transcribed fragments (TDFs) and the sequenced genome of the double haploid- (DH-) Pahang of the malaccensis subspecies that is available in the network. A total of 253 transcript-derived fragments (TDFs) were detected with apparent size of 100-4000 bp using 5 pairs of AFLP primers, of which 21 were differentially expressed during the different stages of banana embryogenesis; 15 of the sequences have matched DH-Pahang chromosomes, with 7 of them being homologous to gene sequences encoding either known or putative protein domains of higher plants. Four TDF sequences were located in all Musa chromosomes, while the rest were located in one or two chromosomes. Their putative individual function is briefly reviewed based on published information, and the potential roles of these genes in embryo development are discussed. Thus the availability of the genome of Musa and the information of TDFs sequences presented here opens new possibilities for an in-depth study of the molecular and biochemical research of zygotic and somatic embryogenesis of Musa.</p>

Alternate JournalScientificWorldJournal
PubMed ID24027442
PubMed Central IDPMC3763263
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Accession Name Pictures Accession number Species / Group SubSpecies / SubGroup Origin Collection Available for distribution
Malaccensis ITC0250 acuminata subsp. malaccensis Unknown ITC