In vitro screening for resistance to Radopholus similis in Musa spp.

TitleIn vitro screening for resistance to Radopholus similis in Musa spp.
Publication TypeJournal Article
Year of Publication2002
AuthorsElsen A, Stoffelen R, Tuyet NThi, Baimey H, de Boulois HDupré, De Waele D
JournalPlant Science
Volume163
Pagination407–416
Abstract

The burrowing nematode, Radopholus similis, is the most important nematode pest of banana and plantain worldwide, causing severe yield losses in commercial and local consumption cultivars. Chemical control is currently the most-used method to manage the nematodes, although nematicides are dangerous, toxic and expensive. Therefore, nematode control through genetic improvement is widely encouraged. Protocols have been set up to detect sources of resistance in the existing Musa germplasm, but the screening under greenhouse and field conditions is laborious and time consuming. In vitro screening could facilitate this process by picking up sources of resistance at an early phase. In this study, a procedure for in vitro screening for the resistance to R. similis was set up by determining the optimal incubation time and the optimal nematode inoculum. In a second step, the optimized procedure was confirmed by screening a selection of Musa varieties with a known host plant response. Except for ‘Yangambi Km5’, their host response under in vitro conditions corresponded to their host response under greenhouse or field conditions. The susceptible status of ‘Grande Naine’, ‘Gros Michel’ and ‘Cachaco’ was confirmed, as well as the resistant status of ‘Pisang Jari Buaya’ and ‘SH-3142’.

DOI10.1016/S0168-9452(02)00129-2
Showing 6 of 6 accessions
Accession Name Pictures Accession number Species / Group SubSpecies / SubGroup Origin Collection Available for distribution
Pisang Jari Buaya ITC0690 AA Unknown Indonesia ITC
Cachaco ITC0643 ABB subgr. Bluggoe Unknown ITC
SH-3142 ITC0425 AA Unknown Honduras ITC
Gros-Michel ITC1122 AAA subgr. Gros Michel Unknown ITC
Yangambi KM5 ITC1123 AAA subgr. Ibota Unknown ITC
Gran Enano
ITC1256 AAA subgr. Cavendish Unknown ITC
Evaluation traits: