Unraveling the complex story of intergenomic recombination in ABB allotriploid bananas

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TitleUnraveling the complex story of intergenomic recombination in ABB allotriploid bananas
Publication TypeJournal Article
Year of Publication2020
AuthorsCenci A, Sardos J, Hueber Y, Martin G, Breton C, Roux N, Swennen R, Carpentier SChristian, Rouard M
JournalAnnals of Botany
Date Published02/2020
Type of Articlejournal arcticle
Keywordsgenetic diversity, homoeologous exchanges, meiosis, Musa, polyploids, subgenomes
Abstract

Bananas (Musa spp.) are a major staple food for hundreds of millions of people in developing countries. The cultivated varieties are seedless and parthenocarpic clones of which the ancestral origin remains to be clarified. The most important cultivars are triploids with an AAA, AAB, or ABB genome constitution, with A and B genomes provided by M. acuminata and M. balbisiana, respectively. Previous studies suggested that inter-genome recombinations were relatively common in banana cultivars and that triploids were more likely to have passed through an intermediate hybrid. In this study, we investigated the chromosome structure within the ABB group, composed of starchy cooking bananas that play an important role in food security.
Methods

Using SNP markers called from RAD-Seq data, we studied the chromosome structure of 36 ABB genotypes spanning defined taxonomic subgroups. To complement our understanding, we search for similar events within nine AB hybrid genotypes.

Recurrent Homoeologous Exchanges (HEs), i.e. chromatin exchanges between A and B subgenomes were unraveled with at least 9 founding events (HE patterns) at the origin of the ABB bananas prior to the clonal diversification. Two independent founding events were found for Pisang Awak genotypes. Two HE patterns, corresponding to genotypes Pelipita and Klue Teparod, show an overrepresentation of B genome contribution. Three HE patterns mainly found in Indian accessions share some recombined regions and two additional patterns did not correspond to any known subgroups.
Conclusions

The discovery of the nine founding events allowed an investigation of the possible routes that led to the creation of the different subgroups, which resulted in new hypotheses. Based on our observations, we suggest different routes that gave rise to the current diversity in the ABB cultivars, routes involving primary AB hybrids, routes leading to shared HEs and routes leading to a B excess ratio. Genetic fluxes took place between M. acuminata and M. balbisiana, particularly in India, where these unbalanced AB hybrids and ABB allotriploids originated, and where cultivated M. balbisiana are abundant. The result of this study clarifies the classification of ABB cultivars, leading possibly to the revision of the classification of this subgroup.

URLhttps://academic.oup.com/aob/advance-article/doi/10.1093/aob/mcaa032/5760888
DOI10.1093/aob/mcaa032
Showing 3 of 43 accessions
Accession Name Pictures Accession number Species / Group SubSpecies / SubGroup Origin Collection Available for distribution
Poovilla Chundan
ITC1752 AB subgr. Kunnan India ITC
Kepok Kuning
ITC1700 ABB subgr. Saba Indonesia ITC
Kepok Tanjung
ITC1745 ABB subgr. Saba Indonesia ITC

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