Panama disease is the most important disease of banana in South Florida. From 1996 to 1998, the performance of 30 dessert and cooking accessions was evaluated at the Tropical Research and Education Center (TREC) of the University of Florida. Field plots were infested, individually, with isolates from three vegetative compatibility groups (VCGs) of the pathogen, Fusarium oxysporum f. sp. cubense, that are found in the area. It was concluded that unfortunately, the most productive and popular varieties were susceptible to disease. The dessert clones 'Pisang Ceylon', 'FHIA01 AAAB', 'FHIA02 AAAA' and 'FHIA17 AAAA', and the cooking clones 'Kumunamba', 'Kandrian' and 'Saba ABB', are recommended for use in all areas in South Florida.