The Musa Genotyping Centre was established at the Laboratory of Molecular Cytogenetics and Cytometry of the Institute of Experimental Botany (IEB), Olomouc, Czech Republic in 2009. A standardized platform for molecular characterization has been developed for Musa germplasm (Christelová et al. 2011). The system is based on 19 microsatellite loci that are scored after the PCR with fluorescently labeled primers and capillary electrophoretic separation with internal standard (GeneScanTM-500 LIZ size standard, Applied Biosystems). The PCR products are multiplexed prior to the separation and loaded onto the automatic 96-capillary ABI 3730xl DNA Analyzer. Electrophoretic separation and signal detection is carried out with default module settings.
Here we provide an Unrooted NJ tree with 221 diploids accessions using 19 SSR markers.
Source of data: Musa Genotyping Centre
Source of Tree: Institute of Experimental Botany (IEB)