|Title||Morphology and reproductive function of induced autotetraploid banana by chromosome doubling|
|Publication Type||Journal Article|
|Year of Publication||2019|
|Authors||POERBA YS, MARTANTI D, HANDAYANI T,|
|Journal||SABRAO Journal of Breeding & Genetics|
Triploid bananas can be generated by crossing between diploid and tetraploid banana cultivars. The production of tetraploid banana plants as candidate parents can be obtained by the application of oryzalin or colchicines. The objectives of this study were to characterize the autotetraploid banana plant “PisangMadu” (Musa, AAAA) induced by in-vitro oryzalin treatment and to use these tetraploid plants as 2x gamete donors in crosses with diploids to generate triploid hybrids. In-vitro shoot cultures of parthenocarpic diploid banana “PisangMadu” (Musa, AA) were initiated and the in vitro shoots were treated by oryzalin at a concentration of 60μM for 7 days in a liquid MS basal medium with addition of 2 mg/l BA. The treated shoots were selected for the solid tetraploids using flowcytometer. Morphology characterization and was study of reproductive function conducted at the Cibinong Science Center for 2 cycles. Fifty two quantitative and qualitative characters were recorded based on UPOV for Banana. Compared with the original diploids, the autotetraploid bananas showed an increase in fruit size and bunch weight. They also showed drooping leaves and truncated fruit apex. Furthermore, the autotetraploids were successfully used as 2x gamete donors in generating triploid hybrids.