Molecular and cytological characterization of the global Musa germplasm collection

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Marker type: 
SSR
Description of the dataset: 

In the present study, flow-cytometric analysis of ploidy level were coupled with the genotyping platform based on SSR markers and analyzed 630 accessions of the genus Musa held at the ITC collection including 49 Reference DNA collection samples (http://www.musanet.org/Musagenotypingcentre/genomicDNA), as well as 27 samples received from Hawaii to enlarge the representation of individual Musa subgroups, and 38 samples collected during the Bioversity International expedition to Indonesia (Sutanto et al. 2016). Altogether, 695 accessions were genotyped, including 327 diploids, 363 triploids and 5 tetraploids.

The extent of genetic diversity among all samples was evaluated using the Nei´s genetic distance coefficient calculation (Nei 1973) and subsequent cluster analysis was done using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA; Michener and Sokal 1957). To enable joint analysis of all ploidy levels (2×, 3× and 4×) the genotypic data was converted into binary (coded by 1/0 = presence/absence) and analyzed as a dominant marker’s record (Weising et al. 2005). Dendrograms were constructed based on the results of UPGMA analysis and visualized in FigTree v1.4.0. The dissimilarity index threshold of 0.25 was used to assess the grouping of the accessions on the dendrogram. Bootstrap support for individual branches was calculated on 1000 replicates and values above 35% (0.35) were used to confirm the fundamental subclustering pattern.

See all accessions used in this study

Showing 40 of 571 accessions
Accession Name Pictures Accession number Species / Group SubSpecies / SubGroup Origin Collectionsort descending Available for distribution
Dwarf Cavendish ITC0002 AAA subgr. Cavendish Unknown ITC
Asamiensa
ITC0007 AAB subgr. Plantain Unknown ITC
Musa velutina ITC0011 velutina subsp. velutina Unknown ITC
Mulolou
ITC0022 AAB subgr. Plantain Gabon ITC
Bungaoisan
ITC0033 AAB subgr. Plantain Indonesia ITC
Daluyao ITC0034 AAB subgr. Plantain Philippines ITC
Muracho ITC0036 AAB Unknown Philippines ITC
Niangafelo ITC0044 AAB subgr. Plantain Unknown ITC
Valery ITC0048 AAA subgr. Cavendish Unknown ITC
Pisang Tongat
ITC0063 AA Unknown Unknown ITC
Type 2 ITC0069 acuminata Unknown Unknown ITC
Tavoy ITC0072 acuminata subsp. burmannica Unknown ITC
Malaccensis
ITC0074 acuminata subsp. malaccensis Unknown ITC
Balbisiana ITC0080 balbisiana Unknown Unknown ITC
Igitsiri (Intuntu) ITC0081 AAA subgr. Mutika/Lujugira Burundi ITC
Tjau Lagada ITC0090 AA Unknown Unknown ITC
Long Tavoy ITC0093 acuminata subsp. burmannica Unknown ITC
Balbisiana (10852) ITC0094 balbisiana Unknown Unknown ITC
Obino l'Ewai ITC0109 AAB subgr. Plantain Unknown ITC
Agbagba ITC0111 AAB subgr. Plantain Unknown ITC
Ntanga 2 ITC0113 AAB subgr. Plantain Unknown ITC
Ubok Iba ITC0114 AAB subgr. Plantain Unknown ITC
Mbang Okon
ITC0118 AAB subgr. Plantain Unknown ITC
Osoaboaso
ITC0119 AAB subgr. Plantain Unknown ITC
Kaamenko
ITC0120 AAB subgr. Plantain Unknown ITC
Ihitisim ITC0121 AAB subgr. Plantain Unknown ITC
Chibulangombe ITC0138 AAA subgr. Mutika/Lujugira Unknown ITC
Nsinabuhaka
ITC0140 AAA subgr. Mutika/Lujugira Unknown ITC
Mzuzu
ITC0144 AAB subgr. Plantain Unknown ITC
Muhongoroka
ITC0156 AAA subgr. Mutika/Lujugira Unknown ITC
One Hand Planty ITC0184 AAB subgr. Plantain Unknown ITC
Ntie ITC0192 AAB subgr. Plantain Unknown ITC
Balbisiana ITC0211 balbisiana Unknown Unknown ITC
Akpakpak ITC0217 AAB subgr. Plantain Unknown ITC
Obubit Ukom
ITC0235 AAB subgr. Plantain Unknown ITC
Safet Velchi ITC0245 AB subgr. Ney Poovan Unknown ITC
Cameroun ITC0246 balbisiana Unknown Unknown ITC
Singapuri
ITC0248 balbisiana Unknown Unknown ITC
Calcutta 4 ITC0249 acuminata subsp. burmannicoides Unknown ITC
Malaccensis ITC0250 acuminata subsp. malaccensis Unknown ITC

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