Molecular and cytological characterization of the global Musa germplasm collection

Publication title or Reference: 

Marker type: 
SSR
Description of the dataset: 

In the present study, flow-cytometric analysis of ploidy level were coupled with the genotyping platform based on SSR markers and analyzed 630 accessions of the genus Musa held at the ITC collection including 49 Reference DNA collection samples (http://www.musanet.org/Musagenotypingcentre/genomicDNA), as well as 27 samples received from Hawaii to enlarge the representation of individual Musa subgroups, and 38 samples collected during the Bioversity International expedition to Indonesia (Sutanto et al. 2016). Altogether, 695 accessions were genotyped, including 327 diploids, 363 triploids and 5 tetraploids.

The extent of genetic diversity among all samples was evaluated using the Nei´s genetic distance coefficient calculation (Nei 1973) and subsequent cluster analysis was done using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA; Michener and Sokal 1957). To enable joint analysis of all ploidy levels (2×, 3× and 4×) the genotypic data was converted into binary (coded by 1/0 = presence/absence) and analyzed as a dominant marker’s record (Weising et al. 2005). Dendrograms were constructed based on the results of UPGMA analysis and visualized in FigTree v1.4.0. The dissimilarity index threshold of 0.25 was used to assess the grouping of the accessions on the dendrogram. Bootstrap support for individual branches was calculated on 1000 replicates and values above 35% (0.35) were used to confirm the fundamental subclustering pattern.

See all accessions used in this study

Showing 40 of 571 accessions
Accession Name Pictures Accession number Species / Group SubSpecies / SubGroup Origin Collection Available for distribution
Losakala
ITC1566 AAB subgr. Plantain Congo (Kinshasa) ITC
Libanga Liboelabokoi
ITC1567 AAB subgr. Plantain Congo (Kinshasa) ITC
Libanga Liboelabokoi
ITC1581 AAB subgr. Plantain Congo (Kinshasa) ITC
Yelola
ITC1582 AAB subgr. Plantain Congo (Kinshasa) ITC
Yumba
ITC1583 AAB subgr. Plantain Congo (Kinshasa) ITC
Banane Tigrée
ITC1615 AAB subgr. Plantain Unknown ITC
Ekona N°1
ITC1616 AAB subgr. Plantain Unknown ITC
Toutoun kelong
ITC1618 AAB subgr. Plantain Unknown ITC
Gui
ITC1619 AAB subgr. Plantain Unknown ITC
Mbouroukou N°2
ITC1620 AAB subgr. Plantain Unknown ITC
Anka
ITC1657 AAB subgr. Plantain Unknown ITC
Atia Ndjokou
ITC1658 AAB subgr. Plantain Unknown ITC
Ban 612
ITC1659 AAB subgr. Plantain Unknown ITC
Bang Make
ITC1660 AAB subgr. Plantain Unknown ITC
Corne 3
ITC1661 AAB subgr. Plantain Unknown ITC
Madre del Plantano
ITC1666 AAB subgr. Plantain Unknown ITC
French Claire Rose
ITC1667 AAB subgr. Plantain Unknown ITC
French Sombre
ITC1668 AAB subgr. Plantain Unknown ITC
Corne Cantebalon
ITC1669 AAB subgr. Plantain Unknown ITC
Madi Madi
ITC1671 AAB subgr. Plantain Unknown ITC
Makemba Noir
ITC1672 AAB subgr. Plantain Unknown ITC
Mbai
ITC1673 AAB subgr. Plantain Unknown ITC
Mbotoko Vert
ITC1674 AAB subgr. Plantain Unknown ITC
Nyombe 3
ITC1675 AAB subgr. Plantain Unknown ITC
Oui
ITC1679 AAB subgr. Plantain Unknown ITC
Nyombe N°1
ITC1709 AAB subgr. Plantain Cameroon ITC
Ban 611
ITC1711 AAB subgr. Plantain Cameroon ITC
Biye
ITC1712 AAB subgr. Plantain Cameroon ITC
Elat Noir
ITC1713 AAB subgr. Plantain Cameroon ITC
Fouem
ITC1714 AAB subgr. Plantain Cameroon ITC
Maqueño
ITC0631 AAB subgr. Plantain Unknown ITC
Musa coccinea
ITC1647 coccinea Unknown Unknown ITC
Kisukari kirefu
ITC1563 Unknown Unknown Tanzania ITC
Musa L. indet. (cultivar)
ITC1533 Unknown Unknown Thailand ITC
Musa itinerans var. itinerans
ITC1571 Unknown Unknown Unknown ITC
Musa yunnanensis
ITC1573 Unknown Unknown Unknown ITC
CIRAD-MU 916
ITC1584 Unknown Unknown Guadeloupe ITC
CIRAD-MU 920
ITC1585 Unknown Unknown Guadeloupe ITC
Kole
ITC1704 Unknown Unknown Unknown ITC
Pagat
ITC1706 Unknown Unknown Unknown ITC

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