Molecular and cytological characterization of the global Musa germplasm collection

Publication title or Reference: 

Marker type: 
SSR
Description of the dataset: 

In the present study, flow-cytometric analysis of ploidy level were coupled with the genotyping platform based on SSR markers and analyzed 630 accessions of the genus Musa held at the ITC collection including 49 Reference DNA collection samples (http://www.musanet.org/Musagenotypingcentre/genomicDNA), as well as 27 samples received from Hawaii to enlarge the representation of individual Musa subgroups, and 38 samples collected during the Bioversity International expedition to Indonesia (Sutanto et al. 2016). Altogether, 695 accessions were genotyped, including 327 diploids, 363 triploids and 5 tetraploids.

The extent of genetic diversity among all samples was evaluated using the Nei´s genetic distance coefficient calculation (Nei 1973) and subsequent cluster analysis was done using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA; Michener and Sokal 1957). To enable joint analysis of all ploidy levels (2×, 3× and 4×) the genotypic data was converted into binary (coded by 1/0 = presence/absence) and analyzed as a dominant marker’s record (Weising et al. 2005). Dendrograms were constructed based on the results of UPGMA analysis and visualized in FigTree v1.4.0. The dissimilarity index threshold of 0.25 was used to assess the grouping of the accessions on the dendrogram. Bootstrap support for individual branches was calculated on 1000 replicates and values above 35% (0.35) were used to confirm the fundamental subclustering pattern.

See all accessions used in this study

Showing 40 of 571 accessions
Accession Name Pictures Accession number Species / Group SubSpecies / SubGroup Origin Collection Available for distribution
Mimbomena
ITC1617 AAB subgr. Plantain Unknown ITC
Penkelong Noir
ITC1621 AAB subgr. Plantain Unknown ITC
Abang Type Somo
ITC1654 AAB subgr. Plantain Unknown ITC
Red Ogoni
ITC1655 AAB subgr. Plantain Unknown ITC
Sima Dikindo
ITC1656 AAB subgr. Plantain Unknown ITC
Douala
ITC1662 AAB subgr. Plantain Unknown ITC
Ebang Mboe
ITC1663 AAB subgr. Plantain Unknown ITC
Ebang Violet
ITC1664 AAB subgr. Plantain Unknown ITC
Ebibi
ITC1665 AAB subgr. Plantain Unknown ITC
Itu Iba Ukom
ITC1670 AAB subgr. Plantain Unknown ITC
Nzorba
ITC1676 AAB subgr. Plantain Unknown ITC
74-79
ITC1677 AAB subgr. Plantain Unknown ITC
Igihobe
ITC1678 AAB subgr. Plantain Unknown ITC
Biya 2
ITC1708 AAB subgr. Plantain Cameroon ITC
76-17
ITC1710 AAB subgr. Plantain Cameroon ITC
One Hand Planty
ITC0184 AAB subgr. Plantain Unknown ITC
Amakake
ITC1484 AAB subgr. Plantain Congo (Kinshasa) ITC
Boofo
ITC1487 AAB subgr. Plantain Congo (Kinshasa) ITC
Amuku
ITC1488 AAB subgr. Plantain Congo (Kinshasa) ITC
Akpasi
ITC1490 AAB subgr. Plantain Congo (Kinshasa) ITC
Adili
ITC1494 AAB subgr. Plantain Congo (Kinshasa) ITC
Bosakaraka
ITC1495 AAB subgr. Plantain Congo (Kinshasa) ITC
Nganganga
ITC1497 AAB subgr. Plantain Congo (Kinshasa) ITC
Nguku
ITC1499 AAB subgr. Plantain Congo (Kinshasa) ITC
Afati 'Wine red'
ITC1501 AAB subgr. Plantain Congo (Kinshasa) ITC
Magoma I
ITC1502 AAB subgr. Plantain Congo (Kinshasa) ITC
Otiti
ITC1503 AAB subgr. Plantain Congo (Kinshasa) ITC
Bolomaise
ITC1504 AAB subgr. Plantain Congo (Kinshasa) ITC
Boosa
ITC1505 AAB subgr. Plantain Congo (Kinshasa) ITC
Afati Black
ITC1506 AAB subgr. Plantain Congo (Kinshasa) ITC
Magoma II
ITC1507 AAB subgr. Plantain Congo (Kinshasa) ITC
Litete
ITC1508 AAB subgr. Plantain Congo (Kinshasa) ITC
Amagaba
ITC1509 AAB subgr. Plantain Congo (Kinshasa) ITC
Yumba Black
ITC1512 AAB subgr. Plantain Congo (Kinshasa) ITC
Inekeleikumi
ITC1513 AAB subgr. Plantain Congo (Kinshasa) ITC
Kambolokoso
ITC1537 AAB subgr. Plantain Congo (Kinshasa) ITC
Aleke
ITC1538 AAB subgr. Plantain Congo (Kinshasa) ITC
Yumba
ITC1565 AAB subgr. Plantain Congo (Kinshasa) ITC
Losakala
ITC1566 AAB subgr. Plantain Congo (Kinshasa) ITC
Libanga Liboelabokoi
ITC1567 AAB subgr. Plantain Congo (Kinshasa) ITC

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