Molecular and cytological characterization of the global Musa germplasm collection

Publication title or Reference: 

Marker type: 
SSR
Description of the dataset: 

In the present study, flow-cytometric analysis of ploidy level were coupled with the genotyping platform based on SSR markers and analyzed 630 accessions of the genus Musa held at the ITC collection including 49 Reference DNA collection samples (http://www.musanet.org/Musagenotypingcentre/genomicDNA), as well as 27 samples received from Hawaii to enlarge the representation of individual Musa subgroups, and 38 samples collected during the Bioversity International expedition to Indonesia (Sutanto et al. 2016). Altogether, 695 accessions were genotyped, including 327 diploids, 363 triploids and 5 tetraploids.

The extent of genetic diversity among all samples was evaluated using the Nei´s genetic distance coefficient calculation (Nei 1973) and subsequent cluster analysis was done using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA; Michener and Sokal 1957). To enable joint analysis of all ploidy levels (2×, 3× and 4×) the genotypic data was converted into binary (coded by 1/0 = presence/absence) and analyzed as a dominant marker’s record (Weising et al. 2005). Dendrograms were constructed based on the results of UPGMA analysis and visualized in FigTree v1.4.0. The dissimilarity index threshold of 0.25 was used to assess the grouping of the accessions on the dendrogram. Bootstrap support for individual branches was calculated on 1000 replicates and values above 35% (0.35) were used to confirm the fundamental subclustering pattern.

See all accessions used in this study

Showing 40 of 571 accessions
Accession Name Pictures Accession number Species / Group SubSpecies / SubGroup Origin Collection Available for distribution
Pama ITC0797 AA Unknown Papua New Guinea ITC
Sihir ITC0810 AA Unknown Papua New Guinea ITC
Kenar ITC0816 AA Unknown Papua New Guinea ITC
Mala ITC0869 AA Unknown Papua New Guinea ITC
Kwosriake ITC0882 AA Unknown Papua New Guinea ITC
Odwa ITC0889 AA Unknown Papua New Guinea ITC
Fu Des ITC0939 AA Unknown Papua New Guinea ITC
Kwaro ITC0943 AA Unknown Papua New Guinea ITC
Wiliman ITC0949 AA Unknown Papua New Guinea ITC
Musa acuminata AAA Umq Bir ITC1743 AAA Unknown Oman ITC
Uzakan ITC0825 AAB Unknown Papua New Guinea ITC
Tonton Kepa ITC0822 AS Unknown Papua New Guinea ITC
Banksii ITC0867 acuminata subsp. banksii Papua New Guinea ITC
Musa acuminata ssp. banksii ITC0879 acuminata subsp. banksii Papua New Guinea ITC
Musa acuminata ssp. banksii ITC0885 acuminata subsp. banksii Papua New Guinea ITC
Cocos ITC0724 AAA subgr. Gros Michel Unknown ITC
Tigua ITC0875 AAB subgr. Iholena Papua New Guinea ITC
Spiral ITC1206 AA Unknown Papua New Guinea ITC
Katual Vunalir ITC0788 AA Unknown Papua New Guinea ITC
Garunga ITC0798 AA Unknown Papua New Guinea ITC
Sepi ITC0849 AA Unknown Papua New Guinea ITC
Wikago ITC0888 AA Unknown Papua New Guinea ITC
Adina ITC0893 AA Unknown Papua New Guinea ITC
Banksii ITC1219 acuminata subsp. banksii Papua New Guinea ITC
Rukumamb Tambey ITC0830 AAB subgr. Iholena Papua New Guinea ITC
Hawain 3 ITC0602 acuminata subsp. banksii Papua New Guinea ITC
Mai'a popo'ulu moa ITC1169 AAB subgr. Maia Maoli/Popoulu Unknown ITC
Gorop ITC0778 AA Unknown Papua New Guinea ITC
Enar ITC0818 AA Unknown Papua New Guinea ITC
Kuspaka ITC0840 AA Unknown Papua New Guinea ITC
Awondaeke ITC0884 AA Unknown Papua New Guinea ITC
Yapu Yapu ITC0923 AA Unknown Papua New Guinea ITC
Hawain 2 ITC0616 acuminata subsp. banksii Papua New Guinea ITC
Banksii ITC0620 acuminata subsp. banksii Papua New Guinea ITC
Banksii ITC0806 acuminata subsp. banksii Papua New Guinea ITC
Akpakpak ITC0217 AAB subgr. Plantain Unknown ITC
Obubit Ntanga green mutant ITC0519 AAB subgr. Plantain Unknown ITC
Meinje ITC1015 AA Unknown Papua New Guinea ITC
Mapua ITC1244 AA Unknown Papua New Guinea ITC
Papat ITC1245 AA Unknown Papua New Guinea ITC

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