Molecular and cytological characterization of the global Musa germplasm collection

Publication title or Reference: 

Marker type: 
SSR
Description of the dataset: 

In the present study, flow-cytometric analysis of ploidy level were coupled with the genotyping platform based on SSR markers and analyzed 630 accessions of the genus Musa held at the ITC collection including 49 Reference DNA collection samples (http://www.musanet.org/Musagenotypingcentre/genomicDNA), as well as 27 samples received from Hawaii to enlarge the representation of individual Musa subgroups, and 38 samples collected during the Bioversity International expedition to Indonesia (Sutanto et al. 2016). Altogether, 695 accessions were genotyped, including 327 diploids, 363 triploids and 5 tetraploids.

The extent of genetic diversity among all samples was evaluated using the Nei´s genetic distance coefficient calculation (Nei 1973) and subsequent cluster analysis was done using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA; Michener and Sokal 1957). To enable joint analysis of all ploidy levels (2×, 3× and 4×) the genotypic data was converted into binary (coded by 1/0 = presence/absence) and analyzed as a dominant marker’s record (Weising et al. 2005). Dendrograms were constructed based on the results of UPGMA analysis and visualized in FigTree v1.4.0. The dissimilarity index threshold of 0.25 was used to assess the grouping of the accessions on the dendrogram. Bootstrap support for individual branches was calculated on 1000 replicates and values above 35% (0.35) were used to confirm the fundamental subclustering pattern.

See all accessions used in this study

Showing 40 of 571 accessions
Accession Name Pictures Accession number Species / Group SubSpecies / SubGroup Origin Collection Available for distribution
Net Pyar
ITC1733 Unknown Unknown Myanmar ITC
Mlambichi
ITC1547 Unknown Unknown Tanzania ITC
Ziwa la mwali
ITC1551 Unknown Unknown Tanzania ITC
Thee Hmwe Wet Ma Lut
ITC1754 Unknown Unknown Myanmar ITC
Khun Sein
ITC1756 Unknown Unknown Myanmar ITC
Hsin An
ITC1757 Unknown Unknown Myanmar ITC
Thu Ngel Sar
ITC1758 Unknown Unknown Myanmar ITC
Hnget Pyaw Minn (Cho Chin 2)
ITC1759 Unknown Unknown Myanmar ITC
Phee Hsone
ITC1767 Unknown Unknown Myanmar ITC
Tongkat Langit Papua
ITC1716 Fe'i Unknown Indonesia ITC
Kawaputa
ITC0927 Fe'i Unknown Papua New Guinea ITC
IBP 5-61
ITC1478 AAA subgr. Gros Michel Cuba ITC
IBP 12
ITC1480 AAA subgr. Gros Michel Cuba ITC
Musa itinerans var. Indet. 'Burmese Blue'
ITC1523 itinerans Unknown Thailand ITC
Musa itinerans var. Xishuangbannaensis
ITC1526 itinerans Unknown China ITC
Balonkawe
ITC0473 ABB subgr. Klue Teparod Philippines ITC
Kunnan
ITC1638 AB subgr. Kunnan India ITC
Padali Moongil
ITC1729 AB subgr. Kunnan India ITC
Agniswar
ITC1747 AB subgr. Kunnan India ITC
Adukka Kunnan
ITC1751 AB subgr. Kunnan India ITC
Poovilla Chundan
ITC1752 AB subgr. Kunnan India ITC
Laknau
ITC0332 AAB subgr. Laknao Philippines ITC
Musa laterita
ITC1521 laterita Unknown Thailand ITC
Musa laterita
ITC1575 laterita Unknown Unknown ITC
Lep Chang Kut
ITC0647 BBB subgr. Lep Chang Kut Unknown ITC
Musa maclayi
ITC1207 maclayi subsp. maclayi Papua New Guinea ITC
Musa maclayi type Hung Si
ITC0614 maclayi subsp. maclayi Papua New Guinea ITC
Poingo
ITC1327 AAB subgr. Maia Maoli/Popoulu Unknown ITC
CIRAD 930
ITC1511 acuminata subsp. malaccensis Guadeloupe ITC
Madang
ITC0254 acuminata subsp. malaccensis Unknown ITC
Malaccensis
ITC0074 acuminata subsp. malaccensis Unknown ITC
Musa mannii
ITC1574 mannii Unknown Unknown ITC
Kachkel
ITC1605 ABB subgr. Monthan India ITC
Kallu Monthan
ITC1609 ABB subgr. Monthan India ITC
Sommarani Monthan
ITC1636 ABB subgr. Monthan India ITC
Pacha Bontha Batheesa
ITC1722 ABB subgr. Monthan India ITC
Monthan
ITC1724 ABB subgr. Monthan India ITC
Booditha Bontha Batheesa
ITC1730 ABB subgr. Monthan India ITC
Musa monticola
ITC1528 monticola Unknown Malaysia ITC
Mlelembo
ITC1544 AA subgr. Mshare Tanzania ITC

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