Molecular and cytological characterization of the global Musa germplasm collection

Publication title or Reference: 

Marker type: 
SSR
Description of the dataset: 

In the present study, flow-cytometric analysis of ploidy level were coupled with the genotyping platform based on SSR markers and analyzed 630 accessions of the genus Musa held at the ITC collection including 49 Reference DNA collection samples (http://www.musanet.org/Musagenotypingcentre/genomicDNA), as well as 27 samples received from Hawaii to enlarge the representation of individual Musa subgroups, and 38 samples collected during the Bioversity International expedition to Indonesia (Sutanto et al. 2016). Altogether, 695 accessions were genotyped, including 327 diploids, 363 triploids and 5 tetraploids.

The extent of genetic diversity among all samples was evaluated using the Nei´s genetic distance coefficient calculation (Nei 1973) and subsequent cluster analysis was done using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA; Michener and Sokal 1957). To enable joint analysis of all ploidy levels (2×, 3× and 4×) the genotypic data was converted into binary (coded by 1/0 = presence/absence) and analyzed as a dominant marker’s record (Weising et al. 2005). Dendrograms were constructed based on the results of UPGMA analysis and visualized in FigTree v1.4.0. The dissimilarity index threshold of 0.25 was used to assess the grouping of the accessions on the dendrogram. Bootstrap support for individual branches was calculated on 1000 replicates and values above 35% (0.35) were used to confirm the fundamental subclustering pattern.

See all accessions used in this study

Showing 40 of 571 accessions
Accession Name Pictures Accession number Species / Group SubSpecies / SubGroup Origin Collection Available for distribution
Butuhan
ITC1074 balbisiana x textilis Unknown Unknown ITC
Banksii
ITC1216 acuminata subsp. banksii Papua New Guinea ITC
Musa schizocarpa?
ITC1218 acuminata subsp. banksii Papua New Guinea ITC
Banksii
ITC0466 acuminata subsp. banksii Unknown ITC
Banksii
ITC0467 acuminata subsp. banksii Unknown ITC
Hawain 3
ITC0617 acuminata subsp. banksii Papua New Guinea ITC
Balbisiana tetraploide
ITC0454 BBB Unknown Honduras ITC
Musa Beccarii
ITC1516 beccarii Unknown Unknown ITC
Musa beccarii var. Hottana
ITC1529 beccarii Unknown Malaysia ITC
Birbutia
ITC1607 ABB subgr. Bluggoe India ITC
Gauria
ITC1765 ABB subgr. Bluggoe India ITC
Sambrani Monthan
ITC1728 ABB subgr. Bontha India ITC
Ney Vannan
ITC1750 ABB subgr. Bontha India ITC
Musa borneensis
ITC1531 borneensis Unknown Malaysia ITC
Musa viridis
ITC1525 Unknown Unknown Vietnam ITC
Musa exotica
ITC1532 Unknown Unknown Vietnam ITC
Musa barioensis
ITC1568 Unknown Unknown Unknown ITC
Musa campestris var. Sarawakensis
ITC1517 campestris Unknown Malaysia ITC
Musa campestris var. Limbangensis
ITC1535 campestris Unknown Brunei ITC
Mtwishe
ITC1540 AAA subgr. Cavendish Tanzania ITC
Grande Naine
ITC1586 AAA subgr. Cavendish Unknown ITC
GCTCV-105
ITC1596 AAA subgr. Cavendish Taiwan ITC
Formosana
ITC1597 AAA subgr. Cavendish Taiwan ITC
Borjahaji
ITC1648 AAA subgr. Cavendish India ITC
Dwarf cavendish
ITC1720 AAA subgr. Cavendish India ITC
Gandevi selection
ITC1726 AAA subgr. Cavendish India ITC
Giant Cavendish
ITC0346 AAA subgr. Cavendish Unknown ITC
Pisang Umbuk
ITC0686 AAA subgr. Cavendish Indonesia ITC
M. cheesmanii
ITC1519 cheesmani Unknown Unknown ITC
Lingu
ITC1485 AAB subgr. Plantain Congo (Kinshasa) ITC
Egbe-o-Mabese
ITC1489 AAB subgr. Plantain Congo (Kinshasa) ITC
Imbelenge
ITC1492 AAB subgr. Plantain Congo (Kinshasa) ITC
Apoka Magbuge
ITC1493 AAB subgr. Plantain Congo (Kinshasa) ITC
Bokpeta
ITC1496 AAB subgr. Plantain Congo (Kinshasa) ITC
Libanga Dark Green
ITC1498 AAB subgr. Plantain Congo (Kinshasa) ITC
Apoka
ITC1510 AAB subgr. Plantain Congo (Kinshasa) ITC
Mimbomena
ITC1617 AAB subgr. Plantain Unknown ITC
Penkelong Noir
ITC1621 AAB subgr. Plantain Unknown ITC
Abang Type Somo
ITC1654 AAB subgr. Plantain Unknown ITC
Red Ogoni
ITC1655 AAB subgr. Plantain Unknown ITC

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