Molecular and cytological characterization of the global Musa germplasm collection

Publication title or Reference: 

Marker type: 
SSR
Description of the dataset: 

In the present study, flow-cytometric analysis of ploidy level were coupled with the genotyping platform based on SSR markers and analyzed 630 accessions of the genus Musa held at the ITC collection including 49 Reference DNA collection samples (http://www.musanet.org/Musagenotypingcentre/genomicDNA), as well as 27 samples received from Hawaii to enlarge the representation of individual Musa subgroups, and 38 samples collected during the Bioversity International expedition to Indonesia (Sutanto et al. 2016). Altogether, 695 accessions were genotyped, including 327 diploids, 363 triploids and 5 tetraploids.

The extent of genetic diversity among all samples was evaluated using the Nei´s genetic distance coefficient calculation (Nei 1973) and subsequent cluster analysis was done using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA; Michener and Sokal 1957). To enable joint analysis of all ploidy levels (2×, 3× and 4×) the genotypic data was converted into binary (coded by 1/0 = presence/absence) and analyzed as a dominant marker’s record (Weising et al. 2005). Dendrograms were constructed based on the results of UPGMA analysis and visualized in FigTree v1.4.0. The dissimilarity index threshold of 0.25 was used to assess the grouping of the accessions on the dendrogram. Bootstrap support for individual branches was calculated on 1000 replicates and values above 35% (0.35) were used to confirm the fundamental subclustering pattern.

See all accessions used in this study

Showing 40 of 571 accessions
Accession Name Pictures Accession number Species / Group SubSpecies / SubGroup Origin Collection Available for distribution
Banksii ITC1219 acuminata subsp. banksii Papua New Guinea ITC
Mai'a popo'ulu moa ITC1169 AAB Unknown Unknown ITC
Gorop ITC0778 AA Unknown Papua New Guinea ITC
Enar ITC0818 AA Unknown Papua New Guinea ITC
Kuspaka ITC0840 AA Unknown Papua New Guinea ITC
Awondaeke ITC0884 AA Unknown Papua New Guinea ITC
Musa acuminata AAA Umq Bir ITC1743 AAA Unknown Oman ITC
Hawain 2 ITC0616 acuminata subsp. banksii Papua New Guinea ITC
Banksii ITC0620 acuminata subsp. banksii Papua New Guinea ITC
Akpakpak ITC0217 AAB subgr. Plantain Unknown ITC
Obubit Ntanga green mutant ITC0519 AAB subgr. Plantain Unknown ITC
Meinje ITC1015 AA Unknown Papua New Guinea ITC
Yalumia ITC1220 AA Unknown Papua New Guinea ITC
Mapua ITC1244 AA Unknown Papua New Guinea ITC
Papat ITC1245 AA Unknown Papua New Guinea ITC
Akondro Mainty ITC0281 AA Unknown Unknown ITC
Waimara ITC0600 AA Unknown Papua New Guinea ITC
Navaradam ITC0770 AA Unknown Papua New Guinea ITC
Himone ITC0886 AA Unknown Papua New Guinea ITC
Grupnai ITC0887 AA Unknown Papua New Guinea ITC
Pai ka ITC0892 AA Unknown Papua New Guinea ITC
Yapu Yapu ITC0923 AA Unknown Papua New Guinea ITC
Horul ITC0870 AAB Unknown Papua New Guinea ITC
Musa acuminata spp. banksii x Musa schizocarpa ITC0858 acuminata x schizocarpa Unknown Papua New Guinea ITC
Banksii ITC0865 acuminata x schizocarpa Unknown Papua New Guinea ITC
Ato ITC0820 AS Unknown Papua New Guinea ITC
Banksii ITC0619 acuminata subsp. banksii Papua New Guinea ITC
Banksii ITC0621 acuminata subsp. banksii Papua New Guinea ITC
Banksii ITC0806 acuminata subsp. banksii Papua New Guinea ITC
Musa acuminata ssp. banksii ITC0885 acuminata subsp. banksii Papua New Guinea ITC
Musa acuminata ssp. banksii ITC0896 acuminata subsp. banksii Papua New Guinea ITC
Banksii ITC0936 acuminata subsp. banksii Papua New Guinea ITC
Banksii ITC0955 acuminata subsp. banksii Papua New Guinea ITC
Musa coccinea ITC0287 coccinea subsp. coccinea Unknown ITC
Menei ITC1021 Fe'i Unknown Papua New Guinea ITC
Utafun ITC0913 Fe'i Unknown Papua New Guinea ITC
Selangor ITC1060 acuminata subsp. malaccensis Unknown ITC
Mbwazirume ITC1356 AAA subgr. Mutika/Lujugira Unknown ITC
Ney Poovan ITC0459 AB subgr. Ney Poovan Unknown ITC
Musa peekelii ssp. peekelii ITC0917 peekelii subsp. peekelii Papua New Guinea ITC

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