Molecular and cytological characterization of the global Musa germplasm collection

Publication title or Reference: 

Marker type: 
SSR
Description of the dataset: 

In the present study, flow-cytometric analysis of ploidy level were coupled with the genotyping platform based on SSR markers and analyzed 630 accessions of the genus Musa held at the ITC collection including 49 Reference DNA collection samples (http://www.musanet.org/Musagenotypingcentre/genomicDNA), as well as 27 samples received from Hawaii to enlarge the representation of individual Musa subgroups, and 38 samples collected during the Bioversity International expedition to Indonesia (Sutanto et al. 2016). Altogether, 695 accessions were genotyped, including 327 diploids, 363 triploids and 5 tetraploids.

The extent of genetic diversity among all samples was evaluated using the Nei´s genetic distance coefficient calculation (Nei 1973) and subsequent cluster analysis was done using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA; Michener and Sokal 1957). To enable joint analysis of all ploidy levels (2×, 3× and 4×) the genotypic data was converted into binary (coded by 1/0 = presence/absence) and analyzed as a dominant marker’s record (Weising et al. 2005). Dendrograms were constructed based on the results of UPGMA analysis and visualized in FigTree v1.4.0. The dissimilarity index threshold of 0.25 was used to assess the grouping of the accessions on the dendrogram. Bootstrap support for individual branches was calculated on 1000 replicates and values above 35% (0.35) were used to confirm the fundamental subclustering pattern.

See all accessions used in this study

Showing 40 of 571 accessions
Accession Name Pictures Accession number Species / Group SubSpecies / SubGroup Origin Collection Available for distribution
Diploide EMBRAPA 205
ITC1193 AA Unknown Brazil ITC
IRFA 905
ITC1267 AA Unknown Nigeria ITC
Bom
ITC1438 AA Unknown Vietnam ITC
Chuoi la rung
ITC1646 AA Unknown Vietnam ITC
Chuoi cau tay
ITC1680 AA Unknown Vietnam ITC
Chuoi bom
ITC1685 AA Unknown Unknown ITC
Keja
ITC1692 AA Unknown Indonesia ITC
Ketan-01
ITC1693 AA Unknown Indonesia ITC
Ayam
ITC1694 AA Unknown Indonesia ITC
Jarum
ITC1695 AA Unknown Unknown ITC
Lidi Bikittinggi
ITC1696 AA Unknown Indonesia ITC
Usim
ITC1697 AA Unknown Indonesia ITC
Sario
ITC1698 AA Unknown Indonesia ITC
Sipulut
ITC1699 AA Unknown Indonesia ITC
Dingin Balige
ITC1707 AA Unknown Unknown ITC
Chuoi cau trang
ITC1717 AA Unknown Unknown ITC
Matti
ITC1761 AA Unknown India ITC
Heva
ITC0257 AA Unknown Unknown ITC
Galeo
ITC0259 AA Unknown Unknown ITC
NBA 14
ITC0267 AA Unknown Unknown ITC
Niyarma Yik
ITC0269 AA Unknown Unknown ITC
Djum Tau
ITC0292 AA Unknown Indonesia ITC
Pitu
ITC0294 AA Unknown Unknown ITC
Guyod
ITC0299 AA Unknown Philippines ITC
Diploid Basilan
ITC0438 AA Unknown Philippines ITC
Morong Principe
ITC0445 AA Unknown Unknown ITC
Pu-te Wey
ITC0447 AA Unknown Malaysia ITC
Pisang Tongat
ITC0063 AA Unknown Unknown ITC
IDO077
ITC0688 AA Unknown Indonesia ITC
Pisang Raja Udang
ITC0976 AA Unknown Unknown ITC
Pisang Sintok
ITC0982 AA Unknown Unknown ITC
Pisang Lemak Manis
ITC1183 AAA Unknown Unknown ITC
Paji
ITC1305 AAA Unknown Tanzania ITC
Mlali
ITC1564 AAA Unknown Tanzania ITC
Hungtu
ITC0372 AAA Unknown Papua New Guinea ITC
Americani
ITC0557 AAA Unknown Unknown ITC
FHIA-23
ITC1265 AAAA Unknown Nigeria ITC
2390-2
ITC0553 AAAA Unknown Jamaica ITC
PA03-22
ITC1261 AAAB Unknown Brazil ITC
FHIA-21(#68)
ITC1332 AAAB Unknown Honduras ITC

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