Molecular and cytological characterization of the global Musa germplasm collection

Publication title or Reference: 

Marker type: 
SSR
Description of the dataset: 

In the present study, flow-cytometric analysis of ploidy level were coupled with the genotyping platform based on SSR markers and analyzed 630 accessions of the genus Musa held at the ITC collection including 49 Reference DNA collection samples (http://www.musanet.org/Musagenotypingcentre/genomicDNA), as well as 27 samples received from Hawaii to enlarge the representation of individual Musa subgroups, and 38 samples collected during the Bioversity International expedition to Indonesia (Sutanto et al. 2016). Altogether, 695 accessions were genotyped, including 327 diploids, 363 triploids and 5 tetraploids.

The extent of genetic diversity among all samples was evaluated using the Nei´s genetic distance coefficient calculation (Nei 1973) and subsequent cluster analysis was done using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA; Michener and Sokal 1957). To enable joint analysis of all ploidy levels (2×, 3× and 4×) the genotypic data was converted into binary (coded by 1/0 = presence/absence) and analyzed as a dominant marker’s record (Weising et al. 2005). Dendrograms were constructed based on the results of UPGMA analysis and visualized in FigTree v1.4.0. The dissimilarity index threshold of 0.25 was used to assess the grouping of the accessions on the dendrogram. Bootstrap support for individual branches was calculated on 1000 replicates and values above 35% (0.35) were used to confirm the fundamental subclustering pattern.

See all accessions used in this study

Showing 40 of 571 accessions
Accession Name Pictures Accession number Species / Group SubSpecies / SubGroup Origin Collection Available for distribution
Saing Todloh ITC0316 AA subgr. Pisang Jari Buaya Unknown ITC
Schizocarpa ITC1002 schizocarpa Unknown Papua New Guinea ITC
Schizocarpa ITC1024 schizocarpa Unknown Papua New Guinea ITC
Banksii No.5 ITC1208 schizocarpa Unknown Papua New Guinea ITC
Mushu ITC0615 schizocarpa Unknown Papua New Guinea ITC
Schizocarpa ITC0852 schizocarpa Unknown Papua New Guinea ITC
Obino l'Ewai ITC0109 AAB subgr. Plantain Unknown ITC
Truncata ITC0393 acuminata subsp. truncata Unknown ITC
Musa velutina ITC0638 velutina subsp. velutina Unknown ITC
Tjau Lagada ITC0090 AA Unknown Unknown ITC
Apindikay ITC0985 AA Unknown Papua New Guinea ITC
Lagun Vunalir ITC0789 AAA Unknown Papua New Guinea ITC
Gebi ITC0877 AAB Unknown Papua New Guinea ITC
Mardape ITC0953 AAB Unknown Papua New Guinea ITC
Japaraka no.1 ITC0604 acuminata x schizocarpa Unknown Papua New Guinea ITC
Musa acuminata ssp. banksii x Musa schizocarpa ITC0815 acuminata x schizocarpa Unknown Papua New Guinea ITC
Sosi ITC0935 acuminata x schizocarpa Unknown Papua New Guinea ITC
Banksii ITC0937 acuminata subsp. banksii Papua New Guinea ITC
Musa boman ITC1026 boman subsp. boman Papua New Guinea ITC
Wain ITC0813 Fe'i Unknown Papua New Guinea ITC
Musa jackeyi ITC0588 jackeyi subsp. jackeyi Unknown ITC
Popoulou (CMR) ITC1135 AAB subgr. Maia Maoli/Popoulu Unknown ITC
Ihi U Maohi ITC0733 AAB subgr. Maia Maoli/Popoulu Unknown ITC
Chuoi Xi Mon ITC1386 AAB subgr. Mysore Vietnam ITC
Pa (Rayong) ITC0672 acuminata subsp. siamea Thailand ITC
Niangafelo ITC0044 AAB subgr. Plantain Unknown ITC
Ntanga 2 ITC0113 AAB subgr. Plantain Unknown ITC
Ntie ITC0192 AAB subgr. Plantain Unknown ITC
Ubok Iba ITC0114 AAB subgr. Plantain Unknown ITC
Ihitisim ITC0121 AAB subgr. Plantain Unknown ITC
Daluyao ITC0034 AAB subgr. Plantain Philippines ITC
Big Ebanga ITC1129 AAB subgr. Plantain Unknown ITC
Njock Kon ITC1133 AAB subgr. Plantain Unknown ITC
Beram ITC0298 AA Unknown Indonesia ITC
Mena ITC0839 AA Unknown Papua New Guinea ITC
Schizocarpa ITC0599 schizocarpa Unknown Papua New Guinea ITC
Schizocarpa ITC0857 schizocarpa Unknown Papua New Guinea ITC
Agbagba ITC0111 AAB subgr. Plantain Unknown ITC
Musa velutina ITC0011 velutina subsp. velutina Unknown ITC
Pogpogon
ITC1184 AA Unknown Unknown ITC

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