In the present study, flow-cytometric analysis of ploidy level were coupled with the genotyping platform based on SSR markers and analyzed 630 accessions of the genus Musa held at the ITC collection including 49 Reference DNA collection samples (http://www.musanet.org/Musagenotypingcentre/genomicDNA), as well as 27 samples received from Hawaii to enlarge the representation of individual Musa subgroups, and 38 samples collected during the Bioversity International expedition to Indonesia (Sutanto et al. 2016). Altogether, 695 accessions were genotyped, including 327 diploids, 363 triploids and 5 tetraploids.
The extent of genetic diversity among all samples was evaluated using the Nei´s genetic distance coefficient calculation (Nei 1973) and subsequent cluster analysis was done using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA; Michener and Sokal 1957). To enable joint analysis of all ploidy levels (2×, 3× and 4×) the genotypic data was converted into binary (coded by 1/0 = presence/absence) and analyzed as a dominant marker’s record (Weising et al. 2005). Dendrograms were constructed based on the results of UPGMA analysis and visualized in FigTree v1.4.0. The dissimilarity index threshold of 0.25 was used to assess the grouping of the accessions on the dendrogram. Bootstrap support for individual branches was calculated on 1000 replicates and values above 35% (0.35) were used to confirm the fundamental subclustering pattern.
See all accessions used in this study
Accession Name | Pictures | Accession number | Species / Group | SubSpecies / SubGroup | Origin | Collection | Available for distribution | |
---|---|---|---|---|---|---|---|---|
Obubit Ntanga green mutant | ITC0519 | AAB | subgr. Plantain | Unknown | ITC |
|
||
Meinje | ITC1015 | AA | Unknown | Papua New Guinea | ITC |
|
||
Mapua | ITC1244 | AA | Unknown | Papua New Guinea | ITC |
|
||
Akondro Mainty | ITC0281 | AA | Unknown | Unknown | ITC |
|
||
Waimara | ITC0600 | AA | Unknown | Papua New Guinea | ITC |
|
||
Navaradam | ITC0770 | AA | Unknown | Papua New Guinea | ITC |
|
||
Grupnai | ITC0887 | AA | Unknown | Papua New Guinea | ITC |
|
||
Pai ka | ITC0892 | AA | Unknown | Papua New Guinea | ITC |
|
||
Horul | ITC0870 | AAB | Unknown | Papua New Guinea | ITC |
|
||
Musa acuminata ssp. banksii x Musa schizocarpa | ITC0815 | acuminata x schizocarpa | Unknown | Papua New Guinea | ITC |
|
||
Musa acuminata spp. banksii x Musa schizocarpa | ITC0858 | acuminata x schizocarpa | Unknown | Papua New Guinea | ITC |
|
||
Banksii | ITC0865 | acuminata x schizocarpa | Unknown | Papua New Guinea | ITC |
|
||
Ato | ITC0820 | AS | Unknown | Papua New Guinea | ITC |
|
||
Banksii | ITC0619 | acuminata | subsp. banksii | Papua New Guinea | ITC |
|
||
Musa acuminata ssp. banksii | ITC0896 | acuminata | subsp. banksii | Papua New Guinea | ITC |
|
||
Banksii | ITC0936 | acuminata | subsp. banksii | Papua New Guinea | ITC |
|
||
Sambrani Monthan | ITC1728 | ABB | subgr. Bontha | India | ITC |
|
||
Ney Vannan | ITC1750 | ABB | subgr. Bontha | India | ITC |
|
||
Musa coccinea | ITC0287 | coccinea | subsp. coccinea | Unknown | ITC |
|
||
Menei | ITC1021 | Fe'i | Unknown | Papua New Guinea | ITC |
|
||
Utafun | ITC0913 | Fe'i | Unknown | Papua New Guinea | ITC |
|
||
Agniswar | ITC1747 | AB | subgr. Kunnan | India | ITC |
|
||
Selangor | ITC1060 | acuminata | subsp. malaccensis | Unknown | ITC |
|
||
Musa peekelii ssp. peekelii | ITC0917 | peekelii | subsp. peekelii | Papua New Guinea | ITC |
|
||
Kambani Zambia/Gurutu/Blokeni | ITC1599 | ABB | subgr. Pisang Awak | Tanzania | ITC |
|
||
Saing Todloh | ITC0316 | AA | subgr. Pisang Jari Buaya | Philippines | ITC |
|
||
INIVIT PB-2003 | ITC1600 | ABB | subgr. Saba | Cuba | ITC |
|
||
Mushu | ITC0615 | schizocarpa | Unknown | Papua New Guinea | ITC |
|
||
Obino l'Ewai | ITC0109 | AAB | subgr. Plantain | Nigeria | ITC |
|
||
Big Ebanga | ITC1129 | AAB | subgr. Plantain | Unknown | ITC |
|
||
Musa velutina | ITC0638 | velutina | subsp. velutina | Unknown | ITC |
|
||
Papat | ITC1245 | AA | Unknown | Papua New Guinea | ITC |
|
||
Himone | ITC0886 | AA | Unknown | Papua New Guinea | ITC |
|
||
Tjau Lagada | ITC0090 | AA | Unknown | Unknown | ITC |
|
||
Lagun Vunalir | ITC0789 | AAA | Unknown | Papua New Guinea | ITC |
|
||
Gebi | ITC0877 | AAB | Unknown | Papua New Guinea | ITC |
|
||
Sosi | ITC0935 | acuminata x schizocarpa | Unknown | Papua New Guinea | ITC |
|
||
Pisang Klutuk Wulung | ITC1587 | balbisiana | Unknown | Unknown | ITC |
|
||
Banksii | ITC0937 | acuminata | subsp. banksii | Papua New Guinea | ITC |
|
||
Musa boman | ITC1026 | boman | subsp. boman | Papua New Guinea | ITC |
|