Effects of N(6)-benzylaminopurine and Indole Acetic Acid on In Vitro Shoot Multiplication, Nodule-like Meristem Proliferation and Plant Regeneration of Malaysian Bananas (Musa spp.).

TitleEffects of N(6)-benzylaminopurine and Indole Acetic Acid on In Vitro Shoot Multiplication, Nodule-like Meristem Proliferation and Plant Regeneration of Malaysian Bananas (Musa spp.).
Publication TypeJournal Article
Year of Publication2012
AuthorsSipen P, Davey MR
JournalTrop Life Sci Res
Volume23
Issue2
Pagination67-80
Date Published2012 Dec
ISSN1985-3718
Abstract

<p>Different concentrations of N(6)-benzylaminopurine (BAP) and indole acetic acid (IAA) in Murashige and Skoog based medium were assessed for their effects on shoot multiplication, nodule-like meristem proliferation and plant regeneration of the Malaysian banana cultivars Pisang Mas, Pisang Nangka, Pisang Berangan and Pisang Awak. BAP at 1-14 mg L(-1) with or without 0.2 mg L(-1) IAA, or BAP at 7-14 mg L(-1) with the same concentration of IAA, was evaluated for shoot multiplication from shoot tips and the proliferation of nodule-like meristems from scalps, respectively. Plant regeneration from scalps was assessed using 1 mg L(-1) BAP and 0.2 mg L(-1) IAA separately, or a combination of these two growth regulators. Data on shoot multiplication, the proliferation of nodule-like meristems with associated plant regeneration were recorded after 30 days of culture. A maximum of 5 shoots per original shoot tip was achieved on medium supplemented with BAP at 5 mg L(-1) (Pisang Nangka), 6 mg L(-1) (Pisang Mas and Pisang Berangan), or 7 mg L(-1) (Pisang Awak), with 0.2 mg L(-1) IAA. BAP at 11 mg L(-1) with 0.2 mg L(-1) IAA induced the most highly proliferating nodule-like meristems in the four banana cultivars. Plant regeneration from scalps was optimum in all cases on medium containing 1 mg L(-1) BAP and 0.2 mg L(-1) IAA. This is the first report on the successful induction of highly proliferating nodule-like meristems and plant regeneration from scalps of the Malaysian banana cultivars Pisang Mas, Pisang Nangka, Pisang Berangan and Pisang Awak.</p>

Alternate JournalTrop Life Sci Res
PubMed ID24575235
PubMed Central IDPMC3799403
Showing 4 of 4 accessions
Accession Name Pictures Accession number Species / Group SubSpecies / SubGroup Origin Collection Available for distribution
Pisang Mas ITC1403 AA subgr. Sucrier Unknown ITC
Pisang Awak ITC0213 ABB subgr. Pisang Awak Unknown ITC
Pisang Nangka ITC0004 AAA-AAB subgr. Pisang Nangka Malaysia ITC
Pisang Berangan
ITC1287 AAA Unknown Unknown ITC